Course detail
Experimental Biophotonics
FSI-TEB-A Acad. year: 2026/2027 Winter semester
Basics of biophotonics including principles of light microscopy, fluorescence, confocal microscopy, quantitative phase imaging especially in holographic microscopy, and polarization microscopy. Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopy observations. Protocols of in vitro investigations of cell behavior. The role of light microscopy techniques for the study of live cells in vitro:
Applications in neurosciences and cancer research.
Demonstrations and practical sections in the laboratory.
This 2/3-day course is also an introduction to the Biophotoniics Core Facility.
Supervisor
Department
Learning outcomes of the course unit
Prerequisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Language of instruction
English
Aims
Specification of controlled education, way of implementation and compensation for absences
The study programmes with the given course
Programme N-PMO-P: Precise Mechanics and Optics, Master's
branch ---: no specialisation, 3 credits, elective
Programme N-FIN-P: Physical Engineering and Nanotechnology, Master's
branch ---: no specialisation, 3 credits, elective
Type of course unit
Lecture
6 hours, optionally
Syllabus
Basic principles of light microscopy for biology
Cell biology and preparation of samples for microscopy
Interferometry and holographic microscopy
Fluorescence, confocal and 2-photon microscopy
Applications in tumor and cell biology
Polarization microscopy and imaging of extracellular matrix
Laboratory exercise
3 hours, compulsory
Syllabus
Lecture themes are developed by laboratory demonstrations and laboratory work of students.