Publication detail

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

BEDNAŘÍK, A. KUBA, P. MOSKOVETS, E. TOMALOVÁ, I. KRÁSENSKÝ, P. HOUŠKA, P. PREISLER, J.

English title

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

Type

WoS Article

Language

en

Original abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in -11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.

Keywords in English

mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput

Released

2014-01-21

Publisher

American Chemical Society

Location

Washington

ISSN

0003-2700

Volume

86

Number

2

Pages from–to

982–986

Pages count

5

BIBTEX


@article{BUT109667,
  author="Antonín {Bednařík} and Jan {Preisler} and Iva {Tomalová} and Eugene {MOSKOVETS} and Pavel {Krásenský} and Pavel {Houška} and Pavel {Kuba}",
  title="Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam",
  journal="ANALYTICAL CHEMISTRY",
  year="2014",
  volume="86",
  number="2",
  pages="982--986",
  doi="10.1021/ac402823n",
  issn="0003-2700"
}