Publication detail

Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

GALANDÁKOVÁ, A. ULRICHOVÁ, J. LANGOVÁ, K. HANÁKOVÁ, A. VRBKA, M. HARTL, M. GALLO, J.

English title

Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

Type

WoS Article

Language

en

Original abstract

Wear testing of total joint replacement (TJR) is mandatory in preclinical testing before implantation of TJR into the human body. Testing is governed by current international standards that recommend bovine serum (BS) as a lubricating fluid to replace synovial fluid (SF). Recently, the use of BS has been criticized because of differences in content, fluid characteristics, and nonhuman origin. As a result, a more realistic lubricant mimicking SF is needed. To define SF composition, we analyzed SF obtained during revisions of total hip and knee arthroplasties and compared it with SF obtained during primary arthroplasties and from patients without TJR. Samples were acquired from 152 patients. We found that the median total protein concentration for all SF was 36.8 mg/mL, which is significantly higher than concentrations currently recommended by the ISO standards. The gama globulin concentration was significantly higher and the phospholipid concentration significantly lower in patients with revision of TJR compared with patients without TJR. No significant difference was found in hyaluronic acid concentration and viscosity among the groups. Our results support the need to improve the definition of a more clinically relevant wear testing lubricant in the ISO standards.

Keywords in English

total hip and knee replacement, protein, hyaluronic acid, phospholipid, viscosity

Released

2016-04-18

Publisher

John Wiley & Sons Inc.

Location

USA

ISSN

1552-4981

Journal

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

Volume

105

Number

6

Pages from–to

1422–1431

Pages count

10

BIBTEX


@article{BUT126663,
  author="GALANDÁKOVÁ, A. and ULRICHOVÁ, J. and LANGOVÁ, K. and HANÁKOVÁ, A. and VRBKA, M. and HARTL, M. and GALLO, J.",
  title="Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments",
  journal="JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS",
  year="2016",
  volume="105",
  number="6",
  pages="1422--1431",
  doi="10.1002/jbm.b.33663",
  issn="1552-4973",
  url="http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33663/full"
}