Publication detail
Measurements of light scattering in an integrated microfluidic waveguide cytometer
SU, X. SINGH, K. CAPJACK, C. PETRÁČEK, J. BACKHOUSE, C.
English title
Measurements of light scattering in an integrated microfluidic waveguide cytometer
Type
Peer-reviewed article not indexed in WoS or Scopus
Language
en
Original abstract
An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.
Keywords in English
light scattering, cytometry, cell analysis, waveguides, Mie theory
Released
2008-03-01
ISSN
1083-3668
Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume
13
Number
2
Pages from–to
024024-1–024024-10
Pages count
10