Publication detail

Gravity-flow open tubular cation chromatography

KUBÁŇ, P. PELCOVÁ, P. KUBÁŇ, V. KLAKURKOVÁ, L. PURNENDU K. D.

English title

Gravity-flow open tubular cation chromatography

Type

Peer-reviewed article not indexed in WoS or Scopus

Language

en

Original abstract

We describe ion chromatography (IC) on open tubular cation exchange columns with a controllable capacity multilayered stationary phase architecture. The columns of relatively large bore (75 lm id) are fabricated by coating fused-silica capillaries with multiple layers of poly(butadiene-maleic acid) (PBMA) copolymer and crosslinking the deposited layers by thermally initiated radical polymerisation. Column capacity increases in a predictable manner with increase in the number of successively coated layers. Gravity flow with a modest head (a2 m) can provide the desired separations within a reasonable period. We provide a minimalist configuration where no suppression is used, the sample is injected hydrodynamically as in CE, and detection is accomplished by an inexpensive homebuilt contactless conductivity detector or a capacitance to voltage digital converter. A 1 m long 75 lm bore column coated with two layers of PBMA allows gravity-flow open tubular IC to separate four alkali cations in a10 min with a 1 mM tartaric acid (TA) eluent. Simultaneous separation of alkali and alkaline earth metal cations can be accomplished in less than 25 min using 1.75 mM pyridinedicarboxylic acid as an eluent. Contactless conductometric detection (C4D) allows LODs down to 150 nmol/L, corresponding to 30 fmol injections. Analysis of real water samples is demonstrated.

Keywords in English

Cations ; Contactless conductometric detection; Gravity-flow; Open tubular ion chromatography

Released

2008-08-20

Publisher

WILEY-VCH Verlag GmbH&Co. KGaA

Location

Weinheim, DE

ISSN

1615-9306

Journal

JOURNAL OF SEPARATION SCIENCE

Volume

2008

Number

31

Pages from–to

2745–2753

Pages count

9

BIBTEX


@article{BUT47036,
  author="Petr {Kubáň} and Pavlína {Pelcová} and Vlastimil {Kubáň} and Lenka {Klakurková} and Dasgupta {Purnendu K.}",
  title="Gravity-flow open tubular cation chromatography",
  journal="JOURNAL OF SEPARATION SCIENCE",
  year="2008",
  volume="2008",
  number="31",
  pages="2745--2753",
  issn="1615-9306"
}