Publication detail

Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy

JANEČKOVÁ, H. VESELÝ, P. CHMELÍK, R.

English title

Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy

Type

WoS Article

Language

en

Original abstract

Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by an acute nutritional and energy deprivation as a survival threat. A phosphate-buffered saline (PBS) survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hours. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal cells. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed an increase in the compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. Studying the PBS survival test with DHM opens a new approach to investigations of the structural integrity of neoplastic cells.

Keywords in English

Tumour cell, nutritional deprivation, energy deprivation, cell survival, cell death, digital holographic microscopy, dynamic phase difference

Released

2009-06-06

Publisher

International Institute of Anticancer Research

Location

Greece

ISSN

0250-7005

Journal

ANTICANCER RESEARCH

Volume

29

Number

6

Pages from–to

2339–2345

Pages count

7

BIBTEX


@article{BUT49714,
  author="Hana {Uhlířová} and Pavel {Veselý} and Radim {Chmelík}",
  title="Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy",
  journal="ANTICANCER RESEARCH",
  year="2009",
  volume="29",
  number="6",
  pages="2339--2345",
  issn="0250-7005",
  url="http://www.iiar-anticancer.org/"
}