Publication detail

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

Vaclav Diopan, Violetta Shestivska, Ondrej Zitka, Michaela Galiova, Vojtech Adam, Jozef Kaiser, Ales Horna, Karel Novotny, Miroslav Liska, Ladislav Havel, Josef Zehnalek, Rene Kizek

English title

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

Type

Peer-reviewed article not indexed in WoS or Scopus

Language

en

Original abstract

The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.

Keywords in English

Thiols, Liquid chromatography, Coulometric detection, Amperometric detection, Heavy metal, Laser induced breakdown spectroscopy

Released

2010-01-12

ISSN

1040-0397

Journal

ELECTROANALYSIS

Volume

22

Number

11

Pages from–to

1248–1259

Pages count

12

BIBTEX


@article{BUT49960,
  author="Jozef {Kaiser} and Miroslav {Liška} and Michaela {Vašinová Galiová}",
  title="Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions",
  journal="ELECTROANALYSIS",
  year="2010",
  volume="22",
  number="11",
  pages="1248--1259",
  issn="1040-0397"
}