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[nazev] => High predation risk decimates survival during the reproduction season
[nazev_orig] => High predation risk decimates survival during the reproduction season
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[popis] => Predators attack conspicuous prey phenotypes that are present in the environment. Male display behavior of conspicuous nuptial coloration becomes risky in the presence of a predator, and adult males face higher predation risk. High predation risk in one sex will lead to low survival and sex ratio bias in adult cohorts, unless the increased predation risk is compensated by higher escape rate. Here, we tested the hypothesis that sand lizards (Lacerta agilis) have sex-specific predation risk and escape rate. We expected the differences to manifest in changes in sex ratio with age, differences in frequency of tail autotomy, and in sex-specific survival rate. We developed a statistical model to estimate predation risk and escape rate, combining the observed sex ratio and frequency of tail autotomy with likelihood-based survival rate. Using Bayesian framework, we estimated the model parameters. We projected the date of the tail autotomy events from growth rates derived from capture-recapture data measurements. We found statistically stable sex ratio in age groups, equal frequency of tail regenerates between sexes, and similar survival rate. Predation risk is similar between sexes, and escape rate increases survival by about 5%. We found low survival rate and a low number of tail autotomy events in females during months when sand lizards mate and lay eggs, indicating high predator pressure throughout reproduction. Our data show that gravid females fail to escape predation. The risks of reproduction season in an ectotherm are a convolution of morphological changes (conspicuous coloration in males and body allometry changes in gravid females), behavior (nuptial displays), and environmental conditions which challenge lizard thermal performance. Performance of endotherm predators in cold spring months endangers gravid females more than displaying males in bright nuptial coloration.
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[citace_text] => SMOLINSKY, R.; HIADLOVSKÁ, Z.; MARŠALA, Š.; ŠKRABÁNEK, P.; ŠKROBÁNEK, M.; MARTÍNKOVÁ, N. High predation risk decimates survival during the reproduction season. Ecology and Evolution, 2022, vol. 12, no. 10, p. 1-12. ISSN: 2045-7758.
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author="Radovan {Smolinsky} and Zuzana {Hiadlovská} and Štěpán {Maršala} and Pavel {Škrabánek} and Michal {Škrobánek} and Natália {Martínková}",
title="High predation risk decimates survival during the reproduction season",
journal="Ecology and Evolution",
year="2022",
volume="12",
number="10",
pages="1--12",
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[popis_en] => Predators attack conspicuous prey phenotypes that are present in the environment. Male display behavior of conspicuous nuptial coloration becomes risky in the presence of a predator, and adult males face higher predation risk. High predation risk in one sex will lead to low survival and sex ratio bias in adult cohorts, unless the increased predation risk is compensated by higher escape rate. Here, we tested the hypothesis that sand lizards (Lacerta agilis) have sex-specific predation risk and escape rate. We expected the differences to manifest in changes in sex ratio with age, differences in frequency of tail autotomy, and in sex-specific survival rate. We developed a statistical model to estimate predation risk and escape rate, combining the observed sex ratio and frequency of tail autotomy with likelihood-based survival rate. Using Bayesian framework, we estimated the model parameters. We projected the date of the tail autotomy events from growth rates derived from capture-recapture data measurements. We found statistically stable sex ratio in age groups, equal frequency of tail regenerates between sexes, and similar survival rate. Predation risk is similar between sexes, and escape rate increases survival by about 5%. We found low survival rate and a low number of tail autotomy events in females during months when sand lizards mate and lay eggs, indicating high predator pressure throughout reproduction. Our data show that gravid females fail to escape predation. The risks of reproduction season in an ectotherm are a convolution of morphological changes (conspicuous coloration in males and body allometry changes in gravid females), behavior (nuptial displays), and environmental conditions which challenge lizard thermal performance. Performance of endotherm predators in cold spring months endangers gravid females more than displaying males in bright nuptial coloration.
[klicova_slova_en] => caudal autotomy; CJS model; escape rate; predation risk; predator pressure; reptile
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[citace_text] => ŠEDA, M. Steiner Tree Problem in Graphs and Mixed Integer Linear Programming-Based Approach in GAMS. WSEAS Transactions on Computers, 2022, vol. 21, no. July, p. 257-262. ISSN: 1109-2750.
[citace_html] => ŠEDA, M. Steiner Tree Problem in Graphs and Mixed Integer Linear Programming-Based Approach in GAMS. WSEAS Transactions on Computers, 2022, vol. 21, no. July, p. 257-262. ISSN: 1109-2750.
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year="2022",
volume="21",
number="July",
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doi="10.37394/23205.2022.21.31",
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url="https://wseas.com/journals/computers/2022/a625105-028(2022).pdf"
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[nazev] => Analysis of the effect of material thickness during the WEDM processon cutting speed, topography and morphology
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[popis] => Wire electrical discharge machining (WEDM) is an unconventional machining technology that is indispensable in many industries. Machining is performed using the thermoelectric principle, while it is possible to machine all at least minimally electrically conductive materials. Due to the wide range of applications of WEDM, it is necessary to ensure the appropriate quality of machined surfaces, regardless of the thickness of the machined materials, while maintaining an acceptable cutting speed. For this purpose, this study was performed to analyse the effect of material thickness on the cutting speed, morphology and topography of Ampcoloy 35 material. In this study, thicknesses from 5 to 160 mm were analysed in 5 mm increments with the same machine parameter settings. The surface topography and morphology were studied using electron and light microscopy, and cross-sections of the sample were created to examine the condition of the subsurface layer. It was found that the values of Ra at the edge ranged from 1.8 to 3.2 µm and in the middle of the sample from 1.7 to 3 µm, while the trend of increasing Ra with thickness is not visible. It is clear from the morphology analysis that a rugged surface with more craters was created at the edges than in the middle of the sample, while segregated lead needles were also formed at the edges of the samples.
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[klicova_slova] => WEDM; electrical discharge machining; thickness; topography; morphology
[klicova_slova_orig] => WEDM; electrical discharge machining; thickness; topography; morphology
[url] => https://doi.org/10.1177/09544054221136516
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[vycet_osob] => MOURALOVÁ, K.; BENEŠ, L.; PROKEŠ, T.; BEDNÁŘ, J.; ZAHRADNÍČEK, R.; FRIES, J.
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[citace_text] => MOURALOVÁ, K.; BENEŠ, L.; PROKEŠ, T.; BEDNÁŘ, J.; ZAHRADNÍČEK, R.; FRIES, J. Analysis of the effect of material thickness during the WEDM processon cutting speed, topography and morphology. PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART B-JOURNAL OF ENGINEERING MANUFACTURE, 2022, vol. 236, no. 15, p. 1-9. ISSN: 0954-4054.
[citace_html] => MOURALOVÁ, K.; BENEŠ, L.; PROKEŠ, T.; BEDNÁŘ, J.; ZAHRADNÍČEK, R.; FRIES, J. Analysis of the effect of material thickness during the WEDM processon cutting speed, topography and morphology. PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART B-JOURNAL OF ENGINEERING MANUFACTURE, 2022, vol. 236, no. 15, p. 1-9. ISSN: 0954-4054.
[citace_rtf] =>
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author="Kateřina {Mouralová} and Libor {Beneš} and Tomáš {Prokeš} and Josef {Bednář} and Radim {Zahradníček} and Jiří {Fries}",
title="Analysis of the effect of material thickness during the WEDM processon cutting speed, topography and morphology",
journal="PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART B-JOURNAL OF ENGINEERING MANUFACTURE",
year="2022",
volume="236",
number="15",
pages="1--9",
doi="10.1177/09544054221136516",
issn="0954-4054",
url="https://doi.org/10.1177/09544054221136516"
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[nazev_en] => Analysis of the effect of material thickness during the WEDM processon cutting speed, topography and morphology
[popis_en] => Wire electrical discharge machining (WEDM) is an unconventional machining technology that is indispensable in many industries. Machining is performed using the thermoelectric principle, while it is possible to machine all at least minimally electrically conductive materials. Due to the wide range of applications of WEDM, it is necessary to ensure the appropriate quality of machined surfaces, regardless of the thickness of the machined materials, while maintaining an acceptable cutting speed. For this purpose, this study was performed to analyse the effect of material thickness on the cutting speed, morphology and topography of Ampcoloy 35 material. In this study, thicknesses from 5 to 160 mm were analysed in 5 mm increments with the same machine parameter settings. The surface topography and morphology were studied using electron and light microscopy, and cross-sections of the sample were created to examine the condition of the subsurface layer. It was found that the values of Ra at the edge ranged from 1.8 to 3.2 µm and in the middle of the sample from 1.7 to 3 µm, while the trend of increasing Ra with thickness is not visible. It is clear from the morphology analysis that a rugged surface with more craters was created at the edges than in the middle of the sample, while segregated lead needles were also formed at the edges of the samples.
[klicova_slova_en] => WEDM; electrical discharge machining; thickness; topography; morphology
[vysledek_datum] => 2022-11-18T00:00:00+01:00
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[nazev] => Část formy vyrobená užitím optimálního nastavení parametrů elektroerozivní drátové řezačky
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[popis] => Funkční vzorek bude uplatněn při reálné výrobě části formy pro vstřikování plastů z měděné slitiny Ampcoloy. Tento výstup představuje finální výstup v řešení projektu a bude implementován do praxe, přičemž přinese výrazné zlepšení v pracovní hygieně obsluze vstřikolisů, kterým nebudou uplívat výrobky ve formě, ale bude možné je snadno odstranit bez použití zdraví neprospěšných separačních sprejů. Dále jejich výroba dle optimálního nastavení parametrů elektroerozivní drátové řezačky přinese ekonomickou úsporu v důsledku snížení strojního času a spotřeby elektrické energie.
[popis_orig] => Funkční vzorek bude uplatněn při reálné výrobě části formy pro vstřikování plastů z měděné slitiny Ampcoloy. Tento výstup představuje finální výstup v řešení projektu a bude implementován do praxe, přičemž přinese výrazné zlepšení v pracovní hygieně obsluze vstřikolisů, kterým nebudou uplívat výrobky ve formě, ale bude možné je snadno odstranit bez použití zdraví neprospěšných separačních sprejů. Dále jejich výroba dle optimálního nastavení parametrů elektroerozivní drátové řezačky přinese ekonomickou úsporu v důsledku snížení strojního času a spotřeby elektrické energie.
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[klicova_slova_orig] => část formy; Ampcoloy; vstřikování plastů; elektroerozivní drátové řezání; snížení strojního času
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[citace_text] => DVOŘÁK, J.;MOURALOVÁ, K.; BEDNÁŘ, J.; PROKEŠ, T.; ZAHRADNÍČEK, R.: Část formy vyrobená užitím optimálního nastavení parametrů elektroerozivní drátové řezačky. (Funkční vzorek)
[citace_html] => DVOŘÁK, J.;MOURALOVÁ, K.; BEDNÁŘ, J.; PROKEŠ, T.; ZAHRADNÍČEK, R.: Část formy vyrobená užitím optimálního nastavení parametrů elektroerozivní drátové řezačky. (Funkční vzorek)
[citace_rtf] =>
[citace_bibtex] => @misc{BUT179989,
author="Kateřina {Mouralová} and Josef {Bednář} and Tomáš {Prokeš} and Radim {Zahradníček}",
title="Část formy vyrobená užitím optimálního nastavení parametrů elektroerozivní drátové řezačky",
year="2022",
note="Functioning sample"
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[nazev_en] => Part of the mold made using the optimal settings of the parameters of the wire electrical discharge machining
[popis_en] => The functional sample will be applied during the actual production of a part of the Ampcoloy copper alloy plastic injection mold. This output represents the final output in the project solution and will be implemented in practice, bringing a significant improvement in the work hygiene of the injection molding operators, who will not spit on the products in the mold, but will be able to easily remove them without using unhealthy separating sprays. Furthermore, their production according to the optimal setting of the parameters of the wire electrical discharge machining will bring economic savings due to a reduction in machine time and electricity consumption.
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[popis] => Semi-permeable barriers to geneflow in principle allow distantly related organisms to capture and exchange pre-adapted genes potentially speeding adaptation. However, describing barriers to geneflow on a genomic scale is non-trivial. We extend classic diagnostic allele counting measures of geneflow across a barrier to the case of genome-scale data. Diagnostic index expectation maximisation (diem) polarises the labelling of bistate markers with respect to the sides of a barrier. An initial state of ignorance is enforced by starting with randomly generated marker polarisations. This means there is no prior on population or taxon membership of the genomes concerned. Using a deterministic data labelling, small numbers of classic diagnostic markers can be replaced by large numbers of markers, each with a diagnostic index. Individuals' hybrid indices (genome admixture proportions) are then calculated genome wide conditioned on marker diagnosticity; within diploid, haplodiploid and/or haploid genome compartments; or indeed over any subset of markers, allowing classical cline width/barrier strength comparisons along genomes. Along-genome barrier strength heterogeneity allows for barrier regions to be identified. Furthermore, blocks of genetic material that have introgressed across a barrier are easily identified with high power. diem indicates panmixis among Myotis myotis bat genomes, with a barrier separating low data quality outliers. In a Mus musculus domesticus/Mus spretus system, diem adds multiple introgressions of olfactory (and vomeronasal) gene clusters in one direction to previous demonstrations of a pesticide resistance gene introgressing in the opposite direction across a strong species barrier. diem is a genome analysis solution, which scales over reduced representation genomics of thousands of markers to treatment of all variant sites in large genomes. While the method lends itself to visualisation, its output of markers with barrier-informative annotation will fuel research in population genetics, phylogenetics and association studies. diem can equip such downstream applications with millions of informative markers.
[popis_orig] => Semi-permeable barriers to geneflow in principle allow distantly related organisms to capture and exchange pre-adapted genes potentially speeding adaptation. However, describing barriers to geneflow on a genomic scale is non-trivial. We extend classic diagnostic allele counting measures of geneflow across a barrier to the case of genome-scale data. Diagnostic index expectation maximisation (diem) polarises the labelling of bistate markers with respect to the sides of a barrier. An initial state of ignorance is enforced by starting with randomly generated marker polarisations. This means there is no prior on population or taxon membership of the genomes concerned. Using a deterministic data labelling, small numbers of classic diagnostic markers can be replaced by large numbers of markers, each with a diagnostic index. Individuals' hybrid indices (genome admixture proportions) are then calculated genome wide conditioned on marker diagnosticity; within diploid, haplodiploid and/or haploid genome compartments; or indeed over any subset of markers, allowing classical cline width/barrier strength comparisons along genomes. Along-genome barrier strength heterogeneity allows for barrier regions to be identified. Furthermore, blocks of genetic material that have introgressed across a barrier are easily identified with high power. diem indicates panmixis among Myotis myotis bat genomes, with a barrier separating low data quality outliers. In a Mus musculus domesticus/Mus spretus system, diem adds multiple introgressions of olfactory (and vomeronasal) gene clusters in one direction to previous demonstrations of a pesticide resistance gene introgressing in the opposite direction across a strong species barrier. diem is a genome analysis solution, which scales over reduced representation genomics of thousands of markers to treatment of all variant sites in large genomes. While the method lends itself to visualisation, its output of markers with barrier-informative annotation will fuel research in population genetics, phylogenetics and association studies. diem can equip such downstream applications with millions of informative markers.
[klicova_slova] => gene capture; geneflow; genome admixture; genome polarisation; hybrid zone; hybridisation; introgression; reduced representation genomics
[klicova_slova_orig] => gene capture; geneflow; genome admixture; genome polarisation; hybrid zone; hybridisation; introgression; reduced representation genomics
[url] => https://besjournals.onlinelibrary.wiley.com/doi/full/10.1111/2041-210X.14010
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[citace_text] => BAIRD, S.; PETRUŽELA, J.; IZAR, J.; ŠKRABÁNEK, P.; MARTÍNKOVÁ, N. Genome polarisation for detecting barriers to geneflow. Methods in Ecology and Evolution, 2022, vol. n/a, no. n/a, p. 1-17. ISSN: 2041-210X.
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author="Stuart J. E. {Baird} and Jan {Petružela} and Jaroň {Izar} and Pavel {Škrabánek} and Natália {Martínková}",
title="Genome polarisation for detecting barriers to geneflow",
journal="Methods in Ecology and Evolution",
year="2022",
volume="n/a",
number="n/a",
pages="1--17",
doi="10.1111/2041-210X.14010",
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[popis_en] => Semi-permeable barriers to geneflow in principle allow distantly related organisms to capture and exchange pre-adapted genes potentially speeding adaptation. However, describing barriers to geneflow on a genomic scale is non-trivial. We extend classic diagnostic allele counting measures of geneflow across a barrier to the case of genome-scale data. Diagnostic index expectation maximisation (diem) polarises the labelling of bistate markers with respect to the sides of a barrier. An initial state of ignorance is enforced by starting with randomly generated marker polarisations. This means there is no prior on population or taxon membership of the genomes concerned. Using a deterministic data labelling, small numbers of classic diagnostic markers can be replaced by large numbers of markers, each with a diagnostic index. Individuals' hybrid indices (genome admixture proportions) are then calculated genome wide conditioned on marker diagnosticity; within diploid, haplodiploid and/or haploid genome compartments; or indeed over any subset of markers, allowing classical cline width/barrier strength comparisons along genomes. Along-genome barrier strength heterogeneity allows for barrier regions to be identified. Furthermore, blocks of genetic material that have introgressed across a barrier are easily identified with high power. diem indicates panmixis among Myotis myotis bat genomes, with a barrier separating low data quality outliers. In a Mus musculus domesticus/Mus spretus system, diem adds multiple introgressions of olfactory (and vomeronasal) gene clusters in one direction to previous demonstrations of a pesticide resistance gene introgressing in the opposite direction across a strong species barrier. diem is a genome analysis solution, which scales over reduced representation genomics of thousands of markers to treatment of all variant sites in large genomes. While the method lends itself to visualisation, its output of markers with barrier-informative annotation will fuel research in population genetics, phylogenetics and association studies. diem can equip such downstream applications with millions of informative markers.
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[nazev_orig] => fuzzyreg: Fuzzy Linear Regression
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[popis] => Estimators for fuzzy linear regression. The functions estimate parameters of fuzzy linear regression models with crisp or fuzzy independent variables (triangular fuzzy numbers are supported). Implements multiple methods for parameter estimation and algebraic operations with triangular fuzzy numbers. Includes functions for summarising, printing and plotting the model fit. Calculates predictions from the model and total error of fit. Diamond (1988) , Hung & Yang (2006) , Lee & Tanaka (1999) , Nasrabadi, Nasrabadi & Nasrabady (2005) , Skrabanek, Marek & Pozdilkova (2021) , Tanaka, Hayashi & Watada (1989) , Zeng, Feng & Li (2017) .
[popis_orig] => Estimators for fuzzy linear regression. The functions estimate parameters of fuzzy linear regression models with crisp or fuzzy independent variables (triangular fuzzy numbers are supported). Implements multiple methods for parameter estimation and algebraic operations with triangular fuzzy numbers. Includes functions for summarising, printing and plotting the model fit. Calculates predictions from the model and total error of fit. Diamond (1988) , Hung & Yang (2006) , Lee & Tanaka (1999) , Nasrabadi, Nasrabadi & Nasrabady (2005) , Skrabanek, Marek & Pozdilkova (2021) , Tanaka, Hayashi & Watada (1989) , Zeng, Feng & Li (2017) .
[klicova_slova] => possibilistic-based fuzzy regression; Fuzzy regression; statistics-based fuzzy regression; R; fuzzy set
[klicova_slova_orig] => possibilistic-based fuzzy regression; Fuzzy regression; statistics-based fuzzy regression; R; fuzzy set
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author="Pavel {Škrabánek} and Natália {Martínková}",
title="fuzzyreg: Fuzzy Linear Regression",
year="2021",
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[nazev_en] => fuzzyreg: Fuzzy Linear Regression
[popis_en] => Estimators for fuzzy linear regression. The functions estimate parameters of fuzzy linear regression models with crisp or fuzzy independent variables (triangular fuzzy numbers are supported). Implements multiple methods for parameter estimation and algebraic operations with triangular fuzzy numbers. Includes functions for summarising, printing and plotting the model fit. Calculates predictions from the model and total error of fit. Diamond (1988) , Hung & Yang (2006) , Lee & Tanaka (1999) , Nasrabadi, Nasrabadi & Nasrabady (2005) , Skrabanek, Marek & Pozdilkova (2021) , Tanaka, Hayashi & Watada (1989) , Zeng, Feng & Li (2017) .
[klicova_slova_en] => possibilistic-based fuzzy regression; Fuzzy regression; statistics-based fuzzy regression; R; fuzzy set
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[popis] => n implementation of TGV methodology published in
TGV searches for an optimal setting of a computer vision system or of its sub-system. Within the system/sub-system must be implemented a weighted means grayscale conversion method. This implementation of TGV is based on one-stage grid-search algorithm supervised by a computer vision expert. The expert assesses settings proposed by the grid-searc method using WECIA graphs. One WECIA graph displays dependence of the system performance on setting of the grayscale conversion weights for one specific setting of the remaining adjustable parameters of the system/sub-system. The performance of the system/sub-system can be evaluated using one or more objective functions where only one of these function is used as a primary objective function, i.e. the grid-search algorihm uses this function for the selection of the optimal parameter setting. The expert can use all the objective functions while assessing a setting proposed by the grid-search algorithm, i.e. nJ WECIA graph are displeyed for one assesed setting where nJ is the number of the objective functions.
[popis_orig] => n implementation of TGV methodology published in
TGV searches for an optimal setting of a computer vision system or of its sub-system. Within the system/sub-system must be implemented a weighted means grayscale conversion method. This implementation of TGV is based on one-stage grid-search algorithm supervised by a computer vision expert. The expert assesses settings proposed by the grid-searc method using WECIA graphs. One WECIA graph displays dependence of the system performance on setting of the grayscale conversion weights for one specific setting of the remaining adjustable parameters of the system/sub-system. The performance of the system/sub-system can be evaluated using one or more objective functions where only one of these function is used as a primary objective function, i.e. the grid-search algorihm uses this function for the selection of the optimal parameter setting. The expert can use all the objective functions while assessing a setting proposed by the grid-search algorithm, i.e. nJ WECIA graph are displeyed for one assesed setting where nJ is the number of the objective functions.
[klicova_slova] => Computer vision; Parameter optimization; Performance evaluationl WECIA graph; Weighted means grayscale conversion
[klicova_slova_orig] => Computer vision; Parameter optimization; Performance evaluationl WECIA graph; Weighted means grayscale conversion
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[citace_rtf] =>
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author="Pavel {Škrabánek} and Natália {Martínková}",
title="TGV methodology MATLAB implementation",
year="2022",
url="https://www.sciencedirect.com/science/article/pii/S0141938222001044#mmc1",
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[popis_en] => n implementation of TGV methodology published in
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[nazev] => Mathematical models for machining optimization of Ampcoloy 35 with different thicknesses using WEDM to improve the surface properties of mould parts
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[popis] => Wire electrical discharge machining (WEDM) is an unconventional machining technology that can be used to machine materials with a minimum electrical conductivity. The technology is often employed in the automotive industry, as it makes it possible to produce mould parts of complex shapes. Copper alloys are commonly used as a electrodes for their high thermal conductivity. The subject of this study was creating mathematical models for machining optimization of Ampcoloy 35 with different thicknesses (ranging from 5 to 160 mm with a step of 5 mm) using WEDM to improve the surface properties of mould parts.The Box-Behnken type experiment was used with a total of 448 samples produced. The following machining parameters were altered over the course of the experiment: Pulse on and off time, Discharge current, and material thickness. The cutting speed was measured and the topography of the machined surfaces in the centre and at the margins of the samples, were analysed. The morphology and subsurface layer were also studied. What makes this study unique is the large number of the tested thicknesses, ranging from 5 to 160 mm with a step of 5 mm. The contribution of this study to the automotive industry and plastic injection mould production is therefore significant. The regression models for cutting speed and surface topography allow for efficient defect-free machining of Ampcoloy 35 of 5-160 mm thicknesses, both in the surface and subsurface layer.
[popis_orig] => Wire electrical discharge machining (WEDM) is an unconventional machining technology that can be used to machine materials with a minimum electrical conductivity. The technology is often employed in the automotive industry, as it makes it possible to produce mould parts of complex shapes. Copper alloys are commonly used as a electrodes for their high thermal conductivity. The subject of this study was creating mathematical models for machining optimization of Ampcoloy 35 with different thicknesses (ranging from 5 to 160 mm with a step of 5 mm) using WEDM to improve the surface properties of mould parts.The Box-Behnken type experiment was used with a total of 448 samples produced. The following machining parameters were altered over the course of the experiment: Pulse on and off time, Discharge current, and material thickness. The cutting speed was measured and the topography of the machined surfaces in the centre and at the margins of the samples, were analysed. The morphology and subsurface layer were also studied. What makes this study unique is the large number of the tested thicknesses, ranging from 5 to 160 mm with a step of 5 mm. The contribution of this study to the automotive industry and plastic injection mould production is therefore significant. The regression models for cutting speed and surface topography allow for efficient defect-free machining of Ampcoloy 35 of 5-160 mm thicknesses, both in the surface and subsurface layer.
[klicova_slova] => WEDM; surface topography; cutting speed; Ampcoloy; design of experiment; machining parameters
[klicova_slova_orig] => WEDM; surface topography; cutting speed; Ampcoloy; design of experiment; machining parameters
[url] => https://www.mdpi.com/1996-1944/16/1/100
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[citace_text] => MOURALOVÁ, K.; BEDNÁŘ, J.; BENEŠ, L.; PROKEŠ, T.; ZAHRADNÍČEK, R.; FRIES, J. Mathematical models for machining optimization of Ampcoloy 35 with different thicknesses using WEDM to improve the surface properties of mould parts. Materials, 2023, vol. 16, no. 1, p. 1-18. ISSN: 1996-1944.
[citace_html] => MOURALOVÁ, K.; BEDNÁŘ, J.; BENEŠ, L.; PROKEŠ, T.; ZAHRADNÍČEK, R.; FRIES, J. Mathematical models for machining optimization of Ampcoloy 35 with different thicknesses using WEDM to improve the surface properties of mould parts. Materials, 2023, vol. 16, no. 1, p. 1-18. ISSN: 1996-1944.
[citace_rtf] =>
[citace_bibtex] => @article{BUT180451,
author="Kateřina {Mouralová} and Josef {Bednář} and Libor {Beneš} and Tomáš {Prokeš} and Radim {Zahradníček} and Jiří {Fries}",
title="Mathematical models for machining optimization of Ampcoloy 35 with different thicknesses using WEDM to improve the surface properties of mould parts
",
journal="Materials",
year="2023",
volume="16",
number="1",
pages="1--18",
doi="10.3390/ma16010100
",
url="https://www.mdpi.com/1996-1944/16/1/100
"
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[nazev_en] => Mathematical models for machining optimization of Ampcoloy 35 with different thicknesses using WEDM to improve the surface properties of mould parts
[popis_en] => Wire electrical discharge machining (WEDM) is an unconventional machining technology that can be used to machine materials with a minimum electrical conductivity. The technology is often employed in the automotive industry, as it makes it possible to produce mould parts of complex shapes. Copper alloys are commonly used as a electrodes for their high thermal conductivity. The subject of this study was creating mathematical models for machining optimization of Ampcoloy 35 with different thicknesses (ranging from 5 to 160 mm with a step of 5 mm) using WEDM to improve the surface properties of mould parts.The Box-Behnken type experiment was used with a total of 448 samples produced. The following machining parameters were altered over the course of the experiment: Pulse on and off time, Discharge current, and material thickness. The cutting speed was measured and the topography of the machined surfaces in the centre and at the margins of the samples, were analysed. The morphology and subsurface layer were also studied. What makes this study unique is the large number of the tested thicknesses, ranging from 5 to 160 mm with a step of 5 mm. The contribution of this study to the automotive industry and plastic injection mould production is therefore significant. The regression models for cutting speed and surface topography allow for efficient defect-free machining of Ampcoloy 35 of 5-160 mm thicknesses, both in the surface and subsurface layer.
[klicova_slova_en] => WEDM; surface topography; cutting speed; Ampcoloy; design of experiment; machining parameters
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[nazev] => Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology
[nazev_orig] => Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology
[duvernost_udaju_id] => S
[popis] => The p53 protein is a key tumor suppressor and the most commonly mutated and down-regulated protein in human tumors. It functions mainly through interaction with DNA, and p53 acts as a transcription factor that recognizes the so-called p53 target sites on the promoters of various genes. P53 has been shown to exist as many isoforms, including three C-terminal isoforms that are produced by alternative splicing. Because the C-terminal domain is responsible for sequence-nonspecific binding and regulation of p53 binding, we have analyzed DNA recognition by these C-terminal isoforms. Using atomic force microscopy, we show for the first time that all C-terminal isoforms recognize superhelical DNA. It is particularly noteworthy that a sequence-specific p53 consensus binding site is bound by p53α and β isoforms with similar affinities, whilst p53α shows higher binding to a quadruplex sequence than both p53β and p53γ, and p53γ loses preferential binding to both the consensus binding sequence and the quadruplex-forming sequence. These results show the important role of the variable p53 C-terminal amino acid sequences for DNA recognition.
[popis_orig] => The p53 protein is a key tumor suppressor and the most commonly mutated and down-regulated protein in human tumors. It functions mainly through interaction with DNA, and p53 acts as a transcription factor that recognizes the so-called p53 target sites on the promoters of various genes. P53 has been shown to exist as many isoforms, including three C-terminal isoforms that are produced by alternative splicing. Because the C-terminal domain is responsible for sequence-nonspecific binding and regulation of p53 binding, we have analyzed DNA recognition by these C-terminal isoforms. Using atomic force microscopy, we show for the first time that all C-terminal isoforms recognize superhelical DNA. It is particularly noteworthy that a sequence-specific p53 consensus binding site is bound by p53α and β isoforms with similar affinities, whilst p53α shows higher binding to a quadruplex sequence than both p53β and p53γ, and p53γ loses preferential binding to both the consensus binding sequence and the quadruplex-forming sequence. These results show the important role of the variable p53 C-terminal amino acid sequences for DNA recognition.
[klicova_slova] => p53 isoforms, G-quadruplex, Atomic force microscopy, p53-DNA binding, Supercoiled DNA
[klicova_slova_orig] => p53 isoforms, G-quadruplex, Atomic force microscopy, p53-DNA binding, Supercoiled DNA
[url] => https://www.sciencedirect.com/science/article/abs/pii/S0300908422003352?via%3Dihub
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[vycet_osob] => GOSWAMI, P.; ŠISLEROVÁ, L.; DOBROVOLNÁ, M.; HAVLÍK, J.; ŠŤASTNÝ, J.; BRÁZDA, V.
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[citace_text] => GOSWAMI, P.; ŠISLEROVÁ, L.; DOBROVOLNÁ, M.; HAVLÍK, J.; ŠŤASTNÝ, J.; BRÁZDA, V. Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology. Biochimie, 2022, vol. 667, no. červen, p. 1-7. ISSN: 1638-6183.
[citace_html] => GOSWAMI, P.; ŠISLEROVÁ, L.; DOBROVOLNÁ, M.; HAVLÍK, J.; ŠŤASTNÝ, J.; BRÁZDA, V. Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology. Biochimie, 2022, vol. 667, no. červen, p. 1-7. ISSN: 1638-6183.
[citace_rtf] =>
[citace_bibtex] => @article{BUT180505,
author="GOSWAMI, P. and ŠISLEROVÁ, L. and DOBROVOLNÁ, M. and HAVLÍK, J. and ŠŤASTNÝ, J. and BRÁZDA, V.",
title="Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology",
journal="Biochimie",
year="2022",
volume="667",
number="červen",
pages="1--7",
doi="10.1016/j.biochi.2022.12.011",
issn="0300-9084",
url="https://www.sciencedirect.com/science/article/abs/pii/S0300908422003352?via%3Dihub"
}
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[nazev_en] => Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology
[popis_en] => The p53 protein is a key tumor suppressor and the most commonly mutated and down-regulated protein in human tumors. It functions mainly through interaction with DNA, and p53 acts as a transcription factor that recognizes the so-called p53 target sites on the promoters of various genes. P53 has been shown to exist as many isoforms, including three C-terminal isoforms that are produced by alternative splicing. Because the C-terminal domain is responsible for sequence-nonspecific binding and regulation of p53 binding, we have analyzed DNA recognition by these C-terminal isoforms. Using atomic force microscopy, we show for the first time that all C-terminal isoforms recognize superhelical DNA. It is particularly noteworthy that a sequence-specific p53 consensus binding site is bound by p53α and β isoforms with similar affinities, whilst p53α shows higher binding to a quadruplex sequence than both p53β and p53γ, and p53γ loses preferential binding to both the consensus binding sequence and the quadruplex-forming sequence. These results show the important role of the variable p53 C-terminal amino acid sequences for DNA recognition.
[klicova_slova_en] => p53 isoforms, G-quadruplex, Atomic force microscopy, p53-DNA binding, Supercoiled DNA
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[popis] => Pyrolysis represents one of the most convenient technologies for the chemical transformation of waste. The exposure to corrosion products and high temperatures does, however, require chemically resistant construction materials. This study was carried out to analyze the corrosion behavior of 1.4571 (AISI 316Ti) and 1.4305 (AISI 303) stainless steels machined with wire electric discharge machining (WEDM) in a pyrolysis environment. Different machining parameters were used for both materials tested to examine the influence of WEDM machining. The total testing time in the pyrolysis environment was 28 days, with the testing chamber being refilled 12 times. The surface topography was analyzed following the WEDM, cleaning, and corrosion test. The surface morphology and cross-section analyses were carried out using electron microscopy at all three stages of the process. An analysis of the chemical composition of the surfaces was carried out as well as of the pyrolysis environment to which the samples were exposed. It was established that the organic acids found in the pyrolysis chamber didnot degrade the tested stainless steels to a meaningful degree. Minor fissures, that is, fine precipitated carbides, were observed on the surface of both the steel types and in their subsurface layer, as well as a significant presence of carbon. This presence wasdirectly connected to the impurities found on the surface after the removal from the test furnace thatwere probably of a protective or passivation nature.
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[klicova_slova] => corrosion; pyrolysis; WEDM; wire electrical discharge machining; stainless steel
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[citace_html] => BENEŠ, L.; MOURALOVÁ, K.; MIDULA, P.; SNOW, J.; LYSOŇKOVÁ, I.; PILNAJ, D.; BURDOVÁ, H.; PROKEŠ, T.; ZAHRADNÍČEK, R.; FRIES, J.; KURÁŇ, P.; KUBĚNKA, M. The Corrosion Behavior of WEDM Machined Stainless Steels in a Pyrolysis Environment. Metals, 2023, vol. 13, no. 1, p. 1-17. ISSN: 2075-4701.
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author="Libor {Beneš} and Kateřina {Mouralová} and Pavol {Midula} and Jan {Snow} and Irena {Lysoňková} and Dominik {Pilnaj} and Hana {Burdová} and Tomáš {Prokeš} and Radim {Zahradníček} and Jiří {Fries} and Pavel {Kuráň} and Martin {Kuběnka}",
title="The Corrosion Behavior of WEDM Machined Stainless Steels in a Pyrolysis Environment",
journal="Metals",
year="2023",
volume="13",
number="1",
pages="1--17",
doi="10.3390/met13010144",
url="https://www.mdpi.com/2075-4701/13/1/144"
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[nazev_en] => The Corrosion Behavior of WEDM Machined Stainless Steels in a Pyrolysis Environment
[popis_en] => Pyrolysis represents one of the most convenient technologies for the chemical transformation of waste. The exposure to corrosion products and high temperatures does, however, require chemically resistant construction materials. This study was carried out to analyze the corrosion behavior of 1.4571 (AISI 316Ti) and 1.4305 (AISI 303) stainless steels machined with wire electric discharge machining (WEDM) in a pyrolysis environment. Different machining parameters were used for both materials tested to examine the influence of WEDM machining. The total testing time in the pyrolysis environment was 28 days, with the testing chamber being refilled 12 times. The surface topography was analyzed following the WEDM, cleaning, and corrosion test. The surface morphology and cross-section analyses were carried out using electron microscopy at all three stages of the process. An analysis of the chemical composition of the surfaces was carried out as well as of the pyrolysis environment to which the samples were exposed. It was established that the organic acids found in the pyrolysis chamber didnot degrade the tested stainless steels to a meaningful degree. Minor fissures, that is, fine precipitated carbides, were observed on the surface of both the steel types and in their subsurface layer, as well as a significant presence of carbon. This presence wasdirectly connected to the impurities found on the surface after the removal from the test furnace thatwere probably of a protective or passivation nature.
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[nazev_orig] => Analysis of the machinability of different types of sintered carbides with WEDM in both water and oil bath
[duvernost_udaju_id] => S
[popis] => The unconventional Wire Electric Discharge Machining (WEDM) technology represents a vital manufacturing technology in different industrial branches. This technology is essential because of the possibility to machine difficult-to-machine materials such as sintered carbides. For this reason, this study analyses the machinability of sintered carbides WKP23S, WSM33S a WK1 with WEDM in both water and oil baths. We investigated the influence of the machining parameters, namely, pulse off time, gap voltage, discharge current, pulse on time, and wire feed, on the cutting speed, surface roughness, and defect occurrence. We investigated 9 different roughness parameters, analysed surface morphology with an electron microscope, and also analysed cross-sectioned samples. We found out that machining sintered carbides in oil bath yields better results than machining in deionized water. The oil tank prevents the removal of the cobalt binder, but it does not reduce fissure occurrence in any significant way. The lowest Ra value, that is 0.7 µm was recorded for the WKP23S sample when machined in oil and Ra 0.9 µm when the same material was machined in water.
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[klicova_slova] => WEDM; cracks; sintered carbide; oil; water; machining parameters
[klicova_slova_orig] => WEDM; cracks; sintered carbide; oil; water; machining parameters
[url] => https://link.springer.com/article/10.1007/s00170-023-10913-4
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author="Kateřina {Mouralová} and Libor {Beneš} and Tomáš {Prokeš} and Radim {Zahradníček} and Jiří {Fries} and Tomáš {Plichta}",
title="Analysis of the machinability of different types of sintered carbides with WEDM in both water and oil bath",
journal="International Journal of Advanced Manufacturing Technology",
year="2023",
volume="124",
number="10",
pages="11",
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issn="0268-3768",
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[nazev_en] => Analysis of the machinability of different types of sintered carbides with WEDM in both water and oil bath
[popis_en] => The unconventional Wire Electric Discharge Machining (WEDM) technology represents a vital manufacturing technology in different industrial branches. This technology is essential because of the possibility to machine difficult-to-machine materials such as sintered carbides. For this reason, this study analyses the machinability of sintered carbides WKP23S, WSM33S a WK1 with WEDM in both water and oil baths. We investigated the influence of the machining parameters, namely, pulse off time, gap voltage, discharge current, pulse on time, and wire feed, on the cutting speed, surface roughness, and defect occurrence. We investigated 9 different roughness parameters, analysed surface morphology with an electron microscope, and also analysed cross-sectioned samples. We found out that machining sintered carbides in oil bath yields better results than machining in deionized water. The oil tank prevents the removal of the cobalt binder, but it does not reduce fissure occurrence in any significant way. The lowest Ra value, that is 0.7 µm was recorded for the WKP23S sample when machined in oil and Ra 0.9 µm when the same material was machined in water.
[klicova_slova_en] => WEDM; cracks; sintered carbide; oil; water; machining parameters
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[id_vav] => 179804
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[2] => Array
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[quotations] => MOURALOVÁ, K.; BENEŠ, L.; PROKEŠ, T.; BEDNÁŘ, J.; ZAHRADNÍČEK, R.; FRIES, J.
[title] => Analysis of the effect of material thickness during the WEDM processon cutting speed, topography and morphology
[typ] => PV
[year] => 2022
[id_vav] => 179862
)
[3] => Array
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[quotations] => DVOŘÁK, J.;MOURALOVÁ, K.; BEDNÁŘ, J.; PROKEŠ, T.; ZAHRADNÍČEK, R.
[title] => Část formy vyrobená užitím optimálního nastavení parametrů elektroerozivní drátové řezačky
[typ] => AV
[year] => 2022
[id_vav] => 179989
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[4] => Array
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[quotations] => BAIRD, S.; PETRUŽELA, J.; IZAR, J.; ŠKRABÁNEK, P.; MARTÍNKOVÁ, N.
[title] => Genome polarisation for detecting barriers to geneflow
[typ] => PV
[year] => 2022
[id_vav] => 180422
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[5] => Array
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[quotations] => ŠKRABÁNEK, P.; MARTÍNKOVÁ, N.
[title] => fuzzyreg: Fuzzy Linear Regression
[typ] => AV
[year] => 2021
[id_vav] => 180423
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[6] => Array
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[quotations] => ŠKRABÁNEK, P.; MARTÍNKOVÁ, N.
[title] => TGV methodology MATLAB implementation
[typ] => AV
[year] => 2022
[id_vav] => 180438
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[7] => Array
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[quotations] => MOURALOVÁ, K.; BEDNÁŘ, J.; BENEŠ, L.; PROKEŠ, T.; ZAHRADNÍČEK, R.; FRIES, J.
[title] => Mathematical models for machining optimization of Ampcoloy 35 with different thicknesses using WEDM to improve the surface properties of mould parts
[typ] => PV
[year] => 2023
[id_vav] => 180451
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[8] => Array
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[quotations] => GOSWAMI, P.; ŠISLEROVÁ, L.; DOBROVOLNÁ, M.; HAVLÍK, J.; ŠŤASTNÝ, J.; BRÁZDA, V.
[title] => Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology
[typ] => PV
[year] => 2022
[id_vav] => 180505
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[quotations] => BENEŠ, L.; MOURALOVÁ, K.; MIDULA, P.; SNOW, J.; LYSOŇKOVÁ, I.; PILNAJ, D.; BURDOVÁ, H.; PROKEŠ, T.; ZAHRADNÍČEK, R.; FRIES, J.; KURÁŇ, P.; KUBĚNKA, M.
[title] => The Corrosion Behavior of WEDM Machined Stainless Steels in a Pyrolysis Environment
[typ] => PV
[year] => 2023
[id_vav] => 180635
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[10] => Array
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[quotations] => MOURALOVÁ, K.; BENEŠ, L.; PROKEŠ, T.; ZAHRADNÍČEK, R.; FRIES, J.; PLICHTA, T.
[title] => Analysis of the machinability of different types of sintered carbides with WEDM in both water and oil bath
[typ] => PV
[year] => 2023
[id_vav] => 181535
)
)
)