Publication detail
Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy
ZIKMUND, T. KVASNICA, L. LOVICAR, L. UHLÍŘOVÁ, H. CHMELÍK, R.
English title
Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy
Type
Paper in proceedings (conference paper)
Language
en
Original abstract
Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.
Keywords in English
digital holographic microscopy, phase image, deformation compensation, least square method
Released
2010-12-14
Publisher
SPIE
ISBN
9780819482365
ISSN
0277-786X
Book
Proceedings of SPIE, Volume 7746
Journal
Proceedings of SPIE
Pages from–to
77460K-1–77460K-7
Pages count
7