Detail publikace

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

ZIKMUND, T. KVASNICA, L. LOVICAR, L. UHLÍŘOVÁ, H. CHMELÍK, R.

Anglický název

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Typ

Stať ve sborníku v databázi WoS či Scopus

Jazyk

en

Originální abstrakt

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Klíčová slova anglicky

digital holographic microscopy, phase image, deformation compensation, least square method

Vydáno

2010-12-14

Nakladatel

SPIE

ISBN

9780819482365

ISSN

0277-786X

Kniha

Proceedings of SPIE, Volume 7746

Časopis

Proceedings of SPIE

Strany od–do

77460K-1–77460K-7

Počet stran

7