Detail publikace

MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

BEDNAŘÍK, A. MACHÁLKOVÁ, M. MOSKOVETS, E. COUFALÍKOVÁ, K. KRÁSENSKÝ, P. HOUŠKA, P. KROUPA, J. NAVRÁTILOVÁ, J. ŠMARDA, J. PREISLER, J.

Anglický název

MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

Typ

Článek WoS

Jazyk

en

Originální abstrakt

The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s. [GRAPHICS] .

Klíčová slova anglicky

Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma

Vydáno

2019-02-15

Nakladatel

SPRINGER

Místo

NEW YORK

ISSN

1044-0305

Časopis

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Ročník

30

Číslo

2

Strany od–do

289–298

Počet stran

10

BIBTEX


@article{BUT162696,
  author="Antonín {Bednařík} and Markéta {Machálková} and Eugene {MOSKOVETS} and Kateřina {Coufalíková} and Pavel {Krásenský} and Pavel {Houška} and Jiří {Kroupa} and Jarmila {Navrátilová} and Jan {Šmarda} and Jan {Preisler}",
  title="MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second",
  journal="JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY",
  year="2019",
  volume="30",
  number="2",
  pages="289--298",
  doi="10.1007/s13361-018-2078-8",
  issn="1044-0305",
  url="https://link.springer.com/article/10.1007/s13361-018-2078-8"
}